The Panhandle Formed by Influenza A and C Virus NS Non-Coding Regions Determines NS Segment Expression

The Panhandle Formed by Influenza A and C Virus NS Non-Coding Regions Determines NS Segment Expression

Exchange of the extremities of the NS segment of type A and C influenza viruses in reverse genetics systems was used to assess their putative role in type specificity. Restoration of each specific proximal panhandle was mandatory to allow the rescue of viruses with heterotypic extremities. Moreover, the transcription level of the modified segment seemed to be directly affected by the distal pan...

1 Binding mode of the activity - modulating C - terminal segment of NS 2 B to NS 3 in the dengue virus NS 2 B - NS 3 protease

Transient expression of coding and non-coding regions of PVY confer resistance to virus infection

One of the most efficient mechanisms by which plants protect themselves from invading virusesis the specific RNA-dependent silencing pathway termed post-transcriptional gene silencing(PTGS). In this mechanism, resistance to a virus is engineered through the expression of asegment of the virus genomein transgenic plants. Potato VirusY (PVY) is one of the mostdamaging viruses of potato, infecting...

H5N1 NS genomic segment distinctly governs the influenza virus infectivity and cytokine induction in monocytic cells

Methods: By reverse genetics, five reassortant influenza viruses carrying the NS genomic segment derived from seasonal influenza A(H1N1), 2009 pandemic A(H1N1), A(H3N2) or H5N1 HPAI virus in the backbone of A/Puerto Rico/8/34 H1N1 (PR8) virus were constructed together with the reassorted PR8 virus control, i.e., rgH1N1sea-NS, rgH1N1pdm-NS, rgH3N2-NS, rgH5N1-NS and rgPR8 viruses, respectively. T...

An NS-segment exonic splicing enhancer regulates influenza A virus replication in mammalian cells

Influenza virus utilizes host splicing machinery to process viral mRNAs expressed from both M and NS segments. Through genetic analysis and functional characterization, we here show that the NS segment of H7N9 virus contains a unique G540A substitution, located within a previously undefined exonic splicing enhancer (ESE) motif present in the NEP mRNA of influenza A viruses. G540A supports virus...